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prsf 1b s15 t79c  (Addgene inc)


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    Structured Review

    Addgene inc prsf 1b s15 t79c
    KEY RESOURCES TABLE
    Prsf 1b S15 T79c, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prsf+1b+s15+t79c/pRSF-1b-S3-M129C+(Plasmid+%23132949)/pmc07006226-642-194-191
    Average 91 stars, based on 1 article reviews
    prsf 1b s15 t79c - by Bioz Stars, 2026-09
    91/100 stars

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    1) Product Images from "Transient protein-RNA interactions guide nascent ribosomal RNA folding"

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding

    Journal: Cell

    doi: 10.1016/j.cell.2019.10.035

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Purification, Labeling, Plasmid Preparation, Software

    Related Articles

    other:

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding
    Article Snippet: Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli BL21-Gold (DE3) Agilent Cat#230132 E. coli Tuner ™ (DE3) Novagen Cat#70623-3 Chemicals, Peptides, and Recombinant Proteins Protocatechuic acid (PCA) Pacific Biosciences Cat#100-215-400 Protocatechuate-3,4-dioxygenase (PCD) Pacific Biosciences Cat#001-028-310 TSY Pacific Biosciences Cat#100-214-900 Cy5 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA25031 Cy5.5 Sulfo-Cyanine5 maleimide Lumiprobe Cat#13380 Cy3 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA23031 E. coli RNA polymerase holoenzyme NEB Cat#M0551S Biolipidure-203 NOF America Corporation Cat#Biolipidure-203 Biolipidure-206 NOF America Corporation Cat#Biolipidure-206 Purified ribosomal proteins of 16S rRNA 3’domain This paper N/A Oligonucleotides Primers, geneBlocks-based DNA transcription templates, labeled DNA oligonucleotides See Data S1 N/A ACU RNA trinucleotide Dharmacon N/A Recombinant DNA pRSF-1b Novagen Cat#71330-3 pRSF-1b-S2 This paper Addgene Plasmid #128590 pRSF-1b-S3 This paper Addgene Plasmid #128591 pRSF-1b-S7-S83C/delta157-178 This paper Addgene Plasmid #128592 pRSF-1b-S10 This paper Addgene Plasmid #128594 pRSF-1b-S13-C85S/P112C This paper Addgene Plasmid #128595 pRSF-1b-S14 This paper Addgene Plasmid #128596 pRSF-1b-S19-T48C This paper Addgene Plasmid #128597 pET-S9 This paper Addgene Plasmid #132948 pRSF-1b-S3-M129C This paper Addgene Plasmid #132949 pRSF-1b-S15-T79C This paper Addgene Plasmid #133048 Software and Algorithms MATLAB R2015a Matworks Inc http://matlab.com Open in a separate window KEY RESOURCES TABLE EXPERIMENTAL MODEL AND SUBJECT DETAILS The genes for the E. coli 30S 3’domain r-proteins were cloned into a pRSF-1b expression vector (Novagen) and overexpressed in BL21-Gold (DE3) (Invitrogen) or Tuner™ (DE3) E. coli strains (Novagen).

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding
    Article Snippet: DNA oligonucleotides (IDT) were labeled at their 3′-end and for some experiments, a quencher was attached to the other terminus ( Zhang et al., 2014 ).

    Plasmid Preparation:

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding
    Article Snippet: pRSF-1b-S3-M129C , This paper , Addgene Plasmid #132949. .. pRSF-1b-S15-T79C , This paper , Addgene Plasmid #133048. ..



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    91
    Addgene inc prsf 1b s15 t79c
    KEY RESOURCES TABLE
    Prsf 1b S15 T79c, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prsf+1b+s15+t79c/pRSF-1b-S3-M129C+(Plasmid+%23132949)/pmc07006226-642-194-191
    Average 91 stars, based on 1 article reviews
    prsf 1b s15 t79c - by Bioz Stars, 2026-09
    91/100 stars
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    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Transient protein-RNA interactions guide nascent ribosomal RNA folding

    doi: 10.1016/j.cell.2019.10.035

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli BL21-Gold (DE3) Agilent Cat#230132 E. coli Tuner ™ (DE3) Novagen Cat#70623-3 Chemicals, Peptides, and Recombinant Proteins Protocatechuic acid (PCA) Pacific Biosciences Cat#100-215-400 Protocatechuate-3,4-dioxygenase (PCD) Pacific Biosciences Cat#001-028-310 TSY Pacific Biosciences Cat#100-214-900 Cy5 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA25031 Cy5.5 Sulfo-Cyanine5 maleimide Lumiprobe Cat#13380 Cy3 Maleimide Mono-Reactive Dye 5-Pack GE Healthcare Cat#PA23031 E. coli RNA polymerase holoenzyme NEB Cat#M0551S Biolipidure-203 NOF America Corporation Cat#Biolipidure-203 Biolipidure-206 NOF America Corporation Cat#Biolipidure-206 Purified ribosomal proteins of 16S rRNA 3’domain This paper N/A Oligonucleotides Primers, geneBlocks-based DNA transcription templates, labeled DNA oligonucleotides See Data S1 N/A ACU RNA trinucleotide Dharmacon N/A Recombinant DNA pRSF-1b Novagen Cat#71330-3 pRSF-1b-S2 This paper Addgene Plasmid #128590 pRSF-1b-S3 This paper Addgene Plasmid #128591 pRSF-1b-S7-S83C/delta157-178 This paper Addgene Plasmid #128592 pRSF-1b-S10 This paper Addgene Plasmid #128594 pRSF-1b-S13-C85S/P112C This paper Addgene Plasmid #128595 pRSF-1b-S14 This paper Addgene Plasmid #128596 pRSF-1b-S19-T48C This paper Addgene Plasmid #128597 pET-S9 This paper Addgene Plasmid #132948 pRSF-1b-S3-M129C This paper Addgene Plasmid #132949 pRSF-1b-S15-T79C This paper Addgene Plasmid #133048 Software and Algorithms MATLAB R2015a Matworks Inc http://matlab.com Open in a separate window KEY RESOURCES TABLE

    Techniques: Recombinant, Purification, Labeling, Plasmid Preparation, Software